PICARD CREATESEQUENCEDICTIONARY

Create a .dict file for a given FASTA file

Example

This wrapper can be used in the following way:

rule create_dict:
    input:
        "genome.fasta",
    output:
        "genome.dict",
    log:
        "logs/picard/create_dict.log",
    params:
        extra="",  # optional: extra arguments for picard.
    # optional specification of memory usage of the JVM that snakemake will respect with global
    # resource restrictions (https://snakemake.readthedocs.io/en/latest/snakefiles/rules.html#resources)
    # and which can be used to request RAM during cluster job submission as `{resources.mem_mb}`:
    # https://snakemake.readthedocs.io/en/latest/executing/cluster.html#job-properties
    resources:
        mem_mb=1024,
    wrapper:
        "v3.8.0-1-g149ef14/bio/picard/createsequencedictionary"

Note that input, output and log file paths can be chosen freely.

When running with

snakemake --use-conda

the software dependencies will be automatically deployed into an isolated environment before execution.

Notes

• The java_opts param allows for additional arguments to be passed to the java compiler, e.g. “-XX:ParallelGCThreads=10” (not for -XmX or -Djava.io.tmpdir, since they are handled automatically).

• The extra param allows for additional program arguments.

• –TMP_DIR is automatically set by resources.tmpdir

• For more information see, https://broadinstitute.github.io/picard/command-line-overview.html#CreateSequenceDictionary

Software dependencies

• picard=3.1.1

• snakemake-wrapper-utils=0.6.2

Input/Output

Input:

• FASTA file

Output:

• .dict file

Authors

• Johannes Köster

Code

__author__ = "Johannes Köster"
__copyright__ = "Copyright 2018, Johannes Köster"
__email__ = "johannes.koester@protonmail.com"
__license__ = "MIT"


import tempfile
from snakemake.shell import shell
from snakemake_wrapper_utils.java import get_java_opts


extra = snakemake.params.get("extra", "")
java_opts = get_java_opts(snakemake)
log = snakemake.log_fmt_shell(stdout=False, stderr=True)

with tempfile.TemporaryDirectory() as tmpdir:
    shell(
        "picard CreateSequenceDictionary"
        " {java_opts} {extra}"
        " --REFERENCE {snakemake.input[0]}"
        " --TMP_DIR {tmpdir}"
        " --OUTPUT {snakemake.output[0]}"
        " {log}"
    )