SAMBAMBA MARKDUP

Marks (default) or removes duplicate reads in BAM file. See details `here https://lomereiter.github.io/sambamba/docs/sambamba-markdup.html`_

Example

This wrapper can be used in the following way:

rule sambamba_markdup:
    input:
        "mapped/{sample}.bam"
    output:
        "mapped/{sample}.rmdup.bam"
    params:
        extra="-r"  # optional parameters
    log:
        "logs/sambamba-markdup/{sample}.log"
    threads: 8
    wrapper:
        "v3.8.0-49-g6f33607/bio/sambamba/markdup"

Note that input, output and log file paths can be chosen freely.

When running with

snakemake --use-conda

the software dependencies will be automatically deployed into an isolated environment before execution.

Software dependencies

• sambamba=1.0

Input/Output

Input:

• bam file

Output:

• deduplicated bam file

Authors

• Jan Forster

Code

__author__ = "Jan Forster"
__copyright__ = "Copyright 2021, Jan Forster"
__email__ = "j.forster@dkfz.de"
__license__ = "MIT"


import os
from snakemake.shell import shell
from tempfile import TemporaryDirectory

log = snakemake.log_fmt_shell(stdout=True, stderr=True)

with TemporaryDirectory() as tempdir:
    shell(
        "sambamba markdup {snakemake.params.extra} --nthreads {snakemake.threads} "
        "--tmpdir {tempdir} {snakemake.input[0]} {snakemake.output[0]} "
        "{log}"
    )