GATK FILTERMUTECTCALLS¶
Run gatk FilterMutectCalls.
URL:
Example¶
This wrapper can be used in the following way:
rule gatk_filtermutectcalls:
input:
vcf="calls/snvs.vcf",
ref="genome.fasta",
output:
vcf="calls/snvs.mutect.filtered.vcf",
log:
"logs/gatk/filter/snvs.log",
params:
extra="--max-alt-allele-count 3", # optional arguments, see GATK docs
java_opts="", # optional
# optional specification of memory usage of the JVM that snakemake will respect with global
# resource restrictions (https://snakemake.readthedocs.io/en/latest/snakefiles/rules.html#resources)
# and which can be used to request RAM during cluster job submission as `{resources.mem_mb}`:
# https://snakemake.readthedocs.io/en/latest/executing/cluster.html#job-properties
resources:
mem_mb=1024,
wrapper:
"v1.1.0/bio/gatk/filtermutectcalls"
Note that input, output and log file paths can be chosen freely.
When running with
snakemake --use-conda
the software dependencies will be automatically deployed into an isolated environment before execution.
Software dependencies¶
gatk4==4.1.4.1
snakemake-wrapper-utils==0.1.3
Notes¶
- The java_opts param allows for additional arguments to be passed to the java compiler, e.g. “-Xmx4G” for one, and “-Xmx4G -XX:ParallelGCThreads=10” for two options.
- The extra param allows for additional program arguments.
- For more information see, https://software.broadinstitute.org/gatk/documentation/article?id=11050
Authors¶
- Patrik Smeds
Code¶
__author__ = "Patrik Smeds"
__copyright__ = "Copyright 2021, Patrik Smeds"
__email__ = "patrik.smeds@gmail.com"
__license__ = "MIT"
from snakemake.shell import shell
from snakemake_wrapper_utils.java import get_java_opts
extra = snakemake.params.get("extra", "")
java_opts = get_java_opts(snakemake)
log = snakemake.log_fmt_shell(stdout=True, stderr=True)
shell(
"gatk --java-options '{java_opts}' FilterMutectCalls "
"-R {snakemake.input.ref} -V {snakemake.input.vcf} "
"{extra} "
"-O {snakemake.output.vcf} "
"{log}"
)