.. _`bio/bedtools/intersect`: INTERSECTBED ============ .. image:: https://img.shields.io/github/issues-pr/snakemake/snakemake-wrappers/bio/bedtools/intersect?label=version%20update%20pull%20requests :target: https://github.com/snakemake/snakemake-wrappers/pulls?q=is%3Apr+is%3Aopen+label%3Abio/bedtools/intersect Intersect BED/BAM/VCF files with bedtools. **URL**: https://bedtools.readthedocs.io/en/latest/content/tools/intersect.html Example ------- This wrapper can be used in the following way: .. code-block:: python rule bedtools_merge: input: left="A.bed", right="B.bed" output: "A_B.intersected.bed" params: ## Add optional parameters extra="-wa -wb" ## In this example, we want to write original entries in A and B for each overlap. log: "logs/intersect/A_B.log" wrapper: "v3.0.1/bio/bedtools/intersect" Note that input, output and log file paths can be chosen freely. When running with .. code-block:: bash snakemake --use-conda the software dependencies will be automatically deployed into an isolated environment before execution. Software dependencies --------------------- * ``bedtools=2.31.1`` Input/Output ------------ **Input:** * ``left``: Path to the left region file. Each feature in left region file is compared to right region(s) file(s) in search of overlaps. (BAM/BED/GFF/VCF formatted) * ``right``: Path or list of paths to region(s) file(s) (BAM/BED/GFF/VCF formatted) **Output:** * Path to the intersection. Params ------ * ``extra``: additional program arguments (except `-a` (left) and `-b` (right)) Authors ------- * Jan Forster Code ---- .. code-block:: python __author__ = "Jan Forster" __copyright__ = "Copyright 2019, Jan Forster" __email__ = "j.forster@dkfz.de" __license__ = "MIT" from snakemake.shell import shell ## Extract arguments extra = snakemake.params.get("extra", "") log = snakemake.log_fmt_shell(stdout=True, stderr=True) shell( "(bedtools intersect" " {extra}" " -a {snakemake.input.left}" " -b {snakemake.input.right}" " > {snakemake.output})" " {log}" ) .. |nl| raw:: html