.. _`bio/cooltools/expected_trans`:

COOLTOOLS EXPECTED_TRANS
========================


.. image:: https://img.shields.io/github/issues-pr/snakemake/snakemake-wrappers/bio/cooltools/expected_trans?label=version%20update%20pull%20requests
   :target: https://github.com/snakemake/snakemake-wrappers/pulls?q=is%3Apr+is%3Aopen+label%3Abio/cooltools/expected_trans

Calculate trans expected for a resolution in an .mcool file

**URL**: https://github.com/open2c/cooltools

Example
-------

This wrapper can be used in the following way:

.. code-block:: python

    rule cooltools_expected_trans:
        input:
            cooler="CN.mm9.1000kb.mcool",  ## Multiresolution cooler file
            view="mm9_view.txt",  ## File with the region names and coordinates
        output:
            "{sample}_{resolution,[0-9]+}.trans.expected.tsv",
        params:
            ## Add optional parameters
            extra="",
        threads: 4
        log:
            "logs/{sample}_{resolution}_trans_expected.log",
        wrapper:
            "v3.0.1/bio/cooltools/expected_trans"

Note that input, output and log file paths can be chosen freely.

When running with

.. code-block:: bash

    snakemake --use-conda

the software dependencies will be automatically deployed into an isolated environment before execution.


Software dependencies
---------------------

* ``cooltools=0.6.1``

Input/Output
------------
**Input:**

* a multiresolution cooler file (.mcool)
* (optional) view, a bed-style file with region coordinates and names to use for analysis

**Output:**

* A .tsv file with mean interaction frequency between chromosomes. Can have a {resolution} wildcard that specifies the resolution for the analysis, then it doesn't need to be specified as a parameter.




Params
------

* ``resolution``: Optional, can be instead specified as a wildcard in the output


* ``extra``: Any additional arguments to pass




Authors
-------

* Ilya Flyamer


Code
----

.. code-block:: python

    __author__ = "Ilya Flyamer"
    __copyright__ = "Copyright 2022, Ilya Flyamer"
    __email__ = "flyamer@gmail.com"
    __license__ = "MIT"

    from snakemake.shell import shell

    ## Extract arguments
    view = snakemake.input.get("view", "")
    if view:
        view = f"--view {view}"
    extra = snakemake.params.get("extra", "")
    log = snakemake.log_fmt_shell(stdout=False, stderr=True)

    resolution = snakemake.params.get(
        "resolution", snakemake.wildcards.get("resolution", 0)
    )
    if not resolution:
        raise ValueError("Please specify resolution either as a wildcard or as a parameter")

    shell(
        "(cooltools expected-trans"
        " {snakemake.input.cooler}::resolutions/{resolution} "
        " {view} "
        " -p {snakemake.threads} "
        " {extra} "
        " -o {snakemake.output}) {log}"
    )


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