.. _`bio/sambamba/slice`: SAMBAMBA SLICE ============== .. image:: https://img.shields.io/github/issues-pr/snakemake/snakemake-wrappers/bio/sambamba/slice?label=version%20update%20pull%20requests :target: https://github.com/snakemake/snakemake-wrappers/pulls?q=is%3Apr+is%3Aopen+label%3Abio/sambamba/slice Fast tool for copying a slice of a BAM file. See details `here https://lomereiter.github.io/sambamba/docs/sambamba-slice.html`_ Example ------- This wrapper can be used in the following way: .. code-block:: python rule sambamba_slice: input: bam="mapped/{sample}.bam", bai="mapped/{sample}.bam.bai" output: "mapped/{sample}.region.bam" params: region="xx:1-10" # region to catch (contig:start-end) log: "logs/sambamba-slice/{sample}.log" wrapper: "v3.0.1/bio/sambamba/slice" Note that input, output and log file paths can be chosen freely. When running with .. code-block:: bash snakemake --use-conda the software dependencies will be automatically deployed into an isolated environment before execution. Software dependencies --------------------- * ``sambamba=1.0`` Input/Output ------------ **Input:** * coordinate-sorted and indexed bam file **Output:** * new bam file with specific region Authors ------- * Jan Forster Code ---- .. code-block:: python __author__ = "Jan Forster" __copyright__ = "Copyright 2021, Jan Forster" __email__ = "j.forster@dkfz.de" __license__ = "MIT" import os from snakemake.shell import shell log = snakemake.log_fmt_shell(stdout=False, stderr=True) shell( "sambamba slice " "{snakemake.input[0]} {snakemake.params.region} > {snakemake.output[0]} " "{log}" ) .. |nl| raw:: html