.. _`bio/samtools/merge`: SAMTOOLS MERGE ============== .. image:: https://img.shields.io/github/issues-pr/snakemake/snakemake-wrappers/bio/samtools/merge?label=version%20update%20pull%20requests :target: https://github.com/snakemake/snakemake-wrappers/pulls?q=is%3Apr+is%3Aopen+label%3Abio/samtools/merge Merge two bam files with samtools. Example ------- This wrapper can be used in the following way: .. code-block:: python rule samtools_merge: input: ["mapped/A.bam", "mapped/B.bam"], output: "merged.bam", log: "merged.log", params: extra="", # optional additional parameters as string threads: 8 wrapper: "v3.0.1/bio/samtools/merge" Note that input, output and log file paths can be chosen freely. When running with .. code-block:: bash snakemake --use-conda the software dependencies will be automatically deployed into an isolated environment before execution. Notes ----- * The `extra` param allows for additional program arguments (not `-@/--threads`, `--write-index`, `-o` or `-O/--output-fmt`). * For more information see, http://www.htslib.org/doc/samtools-merge.html Software dependencies --------------------- * ``samtools=1.18`` * ``snakemake-wrapper-utils=0.6.2`` Input/Output ------------ **Input:** * list of bam files to merge **Output:** * merged bam file Authors ------- * Johannes Köster * Filipe G. Vieira Code ---- .. code-block:: python __author__ = "Johannes Köster" __copyright__ = "Copyright 2016, Johannes Köster" __email__ = "koester@jimmy.harvard.edu" __license__ = "MIT" from snakemake.shell import shell from snakemake_wrapper_utils.samtools import get_samtools_opts samtools_opts = get_samtools_opts(snakemake) extra = snakemake.params.get("extra", "") log = snakemake.log_fmt_shell(stdout=True, stderr=True) shell("samtools merge {samtools_opts} {extra} {snakemake.input} {log}") .. |nl| raw:: html