.. _`bio/samtools/view`: SAMTOOLS VIEW ============= .. image:: https://img.shields.io/github/issues-pr/snakemake/snakemake-wrappers/bio/samtools/view?label=version%20update%20pull%20requests :target: https://github.com/snakemake/snakemake-wrappers/pulls?q=is%3Apr+is%3Aopen+label%3Abio/samtools/view Convert or filter SAM/BAM. **URL**: http://www.htslib.org/doc/samtools-view.html Example ------- This wrapper can be used in the following way: .. code-block:: python rule samtools_view: input: "{sample}.sam", output: bam="{sample}.bam", idx="{sample}.bai", log: "{sample}.log", params: extra="", # optional params string region="", # optional region string threads: 2 wrapper: "v3.0.1/bio/samtools/view" Note that input, output and log file paths can be chosen freely. When running with .. code-block:: bash snakemake --use-conda the software dependencies will be automatically deployed into an isolated environment before execution. Notes ----- * The `extra` param allows for additional program arguments (not `-@/--threads`, `--write-index`, `-o` or `-O/--output-fmt`). * The `region` param allows one to specify region to extract as `RNAME[:STARTPOS[-ENDPOS]]` (e.g. `chr1`, `chr2:10000000`, `chr3:1000-2000`, `'*'`). Software dependencies --------------------- * ``samtools=1.18`` * ``snakemake-wrapper-utils=0.6.2`` Input/Output ------------ **Input:** * SAM/BAM/CRAM file **Output:** * SAM/BAM/CRAM file * SAM/BAM/CRAM index file (optional) Authors ------- * Johannes Köster * Filipe G. Vieira * Lance Parsons Code ---- .. code-block:: python __author__ = "Johannes Köster" __copyright__ = "Copyright 2016, Johannes Köster" __email__ = "koester@jimmy.harvard.edu" __license__ = "MIT" from snakemake.shell import shell from snakemake_wrapper_utils.samtools import get_samtools_opts samtools_opts = get_samtools_opts(snakemake) extra = snakemake.params.get("extra", "") region = snakemake.params.get("region", "") log = snakemake.log_fmt_shell(stdout=True, stderr=True, append=True) shell("samtools view {samtools_opts} {extra} {snakemake.input[0]} {region} {log}") .. |nl| raw:: html