READ_GC

GC content distribution of reads.

URL: https://rseqc.sourceforge.net/#read-gc-py

Example

This wrapper can be used in the following way:

rule test_rseqc_read_gc:
    input:
        "A.bam",
    output:
        xls="A.gc_percent.xls",
        plot_r="A.script.r",
        pdf="A.plot.pdf",
    log:
        "rseqc.log",
    params:
        extra="",
    wrapper:
        "v3.9.0/bio/rseqc/read_gc"

Note that input, output and log file paths can be chosen freely.

When running with

snakemake --use-conda

the software dependencies will be automatically deployed into an isolated environment before execution.

Software dependencies

• rseqc=5.0.3

Input/Output

Input:

• Path to SAM/BAM file

Output:

• xls: Optional path to gc percent table

• plot_r: Optional path to R script

• pdf: Optional path to PDF-formatted graph

Params

• extra: Optional parameters for read_gc.py

Authors

• Thibault Dayris

Code

# coding: utf-8

"""Snakemake wrapper for RSeQC read_GC.py"""

__author__ = "Thibault Dayris"
__mail__ = "thibault.dayris@gustaveroussy.fr"
__copyright__ = "Copyright 2024, Thibault Dayris"
__license__ = "MIT"

from tempfile import TemporaryDirectory
from snakemake import shell

extra = snakemake.params.get("extra", "")
log = snakemake.log_fmt_shell(stdout=True, stderr=True, append=True)


with TemporaryDirectory() as tempdir:
    shell(
        "read_GC.py {extra} "
        "--input-file {snakemake.input} "
        "--out-prefix {tempdir}/out "
        "{log} "
    )

    if "xls" in snakemake.output.keys():
        shell(
            "mv --verbose " "{tempdir}/out.GC.xls " "{snakemake.output.xls} " "{log} "
        )

    if "plot_r" in snakemake.output.keys():
        shell(
            "mv --verbose "
            "{tempdir}/out.GC_plot.r "
            "{snakemake.output.plot_r} "
            "{log} "
        )

    if "pdf" in snakemake.output.keys():
        shell(
            "mv --verbose "
            "{tempdir}/out.GC_plot.pdf "
            "{snakemake.output.pdf} "
            "{log} "
        )